ORA? ROX H實時qPCR探針混合液基于小分子抑制劑技術(shù)熱啟動PCR，允許在標(biāo)準(zhǔn)和快速qPCR循環(huán)條件下實現(xiàn)最高的靈敏度和特異性。它們在AT和GC豐富的模板上都提供了很好的結(jié)果，在復(fù)用和保證快速擴(kuò)展早期Ct值的同時，最少或沒有變化。

ORA? ROX H實時qPCR探針混合液提供了簡化過程跟蹤的另一個優(yōu)點(diǎn)，因為它們用惰性藍(lán)色染料著色，以使樣品在移液和處理過程中更好地可見。

我們的主混合料提供PCR水，以保證最佳性能。以適應(yīng)廣泛的儀器范圍? qPCR探針母料有三種版本-無ROX、低或高ROX濃度。

作為可選用途，將ROX被動參比染料預(yù)先混合在ROX L和ROX H qPCR混合物中。如果買方有一個能夠進(jìn)行可選ROX檢測的儀器，并希望執(zhí)行可選的信號標(biāo)準(zhǔn)化，則用戶必須在軟件中選擇該選項。

買方注意：購買本產(chǎn)品時，不得轉(zhuǎn)讓美國專利5928907和美國以外相應(yīng)專利的任何權(quán)利。

產(chǎn)品適用：

高濃度ROX校準(zhǔn)儀器的qPCR

基于特定探針的qPCR分析：

TaqMan?，分子信標(biāo)，Scorpions?  探頭

基因表達(dá)分析，量化：

gDNA，cDNA，病毒DNA，低拷貝數(shù)基因

產(chǎn)品優(yōu)點(diǎn)：

通用-標(biāo)準(zhǔn)和快速循環(huán)，全探針qPCR分析

GC或AT富模板的qPCR，

單復(fù)和多路復(fù)用快速擴(kuò)張，早期Ct

惰性藍(lán)色染料，提高樣品的可視性和跟蹤性

聲明：產(chǎn)品僅科研使用。

1. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology BMC Genomics, 2017 Nov 3 Luciana Fernandes Brito, Marta Irla, J?rn Kalinowski et al. … inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

2.  Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum Marine Drugs, 2016 Jun 30 Nadja A. Henke, Sabine A. E. Heider, Petra Peters-Wendisch et al. amplified …using All-in HiFi polymerase (highQu, Kraichtal, Germany)...

3.  Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains Industrial Crops and Products, 2017 Nov 1 Marta Irla, Ingemar N?rdal, Trygve Brautaset et al. …The DNA amplification was carried out with ALLin? HiFi DNA Polymerase (highQu)…

4.  Genome-Based Genetic Tool Development for Bacillus methanolicus:Theta- and Rolling Circle-Replication Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production Frontiers in Microbiology, 2016 Sep 22 Marta Irla, Tonje M. B. Heggeset, Ingemar N?rdal, Lidia Paul, Tone Haugen et al. … inserts with ALLinTM HiFi DNA Polymerase (highQu) or the ExpandTM High Fidelity PCR System (Roche)….

5. Programming biological operating systems: Genome design, assembly and activation Nature Methods Vol 11, 2014 Daniel G Gibson …amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

6.  Genomic, transcriptomic and phenotypic analysis of the plant growth promoting and phosphate solubilizing Paenibacillus riograndensis SBR5 Dissertation, Bielefeld University, 2017 Jun Luciana Fernandes de Brito …inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…

7.  Role of adhesin proteins in Chlamydia infection Dissertation, Heinrich Heine University Düsseldorf, 2017 Feb Alison Favaroni …PCR with All-in HiFi Polymerase (highQu)…

8.  Charakterisierung der pH abh?ngigen Konformation und Multimerisierung des Influenza-A-Virus M1-Proteins Dissertation, Universit?t zu Lübeck, 2016 Jun Jens Klingbeil … wurde die PCR-Ans?tze mit der ALLinTM HiFi DNA Polymerase (highQu) nach Herstellerangaben durchgeführt…

9.  Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum Genes, Basel, April 2018 Nadja A. Henke, Julian Wichmann, [...], and Volker F. Wendisch CR-generated fragments (All-in HiFi, highQu, Kraichtal, Germany) were assembled into isolated plasmids

10. Variation in ectomycorrhizal fungal communities associated with Silver linden (Tilia tomentosa) within and across urban areas FEMS Microbiology Ecology, 94, 2018 Maarten Van Geel, KangYu, Tobias Ceulemans 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and 1 μL genomic DNA.

11.  Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus Frontiers in microbiology, 2019 M Gil López, M Irla, LF Brito Inserts were amplified by polymerase chain reactions (PCRs) using ALLin TM HiFi DNA Polymerase (HighQu)

12.  Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas … Front. Bioeng. Biotechnol., 26 September 2019 M Mindt, S Hannibal… PCR-fragments were amplified using ALLin? HiFi DNA Polymerase (highQu GmbH)

13.  Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum Bioresource technology, 2019 M Mindt, M Heuser amplified using ALLin? HiFi DNA Polymerase (highQu GmbH)

14. Forest edge effects on the mycorrhizal communities of the dual-mycorrhizal tree species Alnus glutinosa (L.) Gaertn. Science of the Total Environment, 2019 M Boeraeve, O Honnay, ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany

15.  Soil organic matter rather than ectomycorrhizal diversity is related to urban tree health PloS one, 2019 M Van Geel, K Yu, G Peeters 1× HiFi Buffer, 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany

16. 16. Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From … 2019 - ir.niist.res.in KM Nampoothiri, M Heuser ALLin TM HiFi DNA Polymerase (highQu GmbH, Kraichtal, Germany).

17. Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein Marine drugs, 2019 NA Henke… amplified by a high-fidelity PCR (Allin HiFi, highQu, Kraichtal, Germany)

18.  Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus bioRxiv, 2019 B Delépine, MG Lopez (PCRs) with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany)

19. Metabolic engineering of Corynebacterium glutamicum towards methanol-dependent growth 20182019 - pub.unibielefeld.de G Hennig PCR to amplify DNA-fragments for cloning was performed with Allin HiFi polymerase (HighQu, Kraichtal, Germany) according to the manufacturer’s protocol.

20.  The impact of forest fragmentation on mycorrhizal communities Dissertation, 2019 Margaux Boeraeve DNA. ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany).

21.  SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly Nature Structural & …, 2020 V K?nigs, COF Machado The proofreading thermostable ALLin RPH Polymerase (highQu) was used for the amplification of PCR fragments for cloning.

22.  Carbon Metabolism of Methylotrophic Methanogens and Asgard Archaea in Marine Sediments Dissertation, Uni Bremen, 2019 X Yin PCR was conducted with ALLin RPH polymerase Kit (highQu, Kraichtal, Germany)